Composite

Part:BBa_K322238

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-10-08)


Lac promoter + wild type firefly luciferase (yellow-green colour)

This part is made of the lac promoter followed by wild type firefly luciferase. This part was submitted because BBa_I712019 contains 6 bases upstream of the start codon, which reduce protein expression by increasing distance between the ribosome binding site and the start codon. Thus, bacteria expressing this part should be brighter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1432


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Categories
Parameters
None